R1475-10

Renin R (Renin Receptor, ATPase H+ -transporting lysosomal accessory interacting protein 2, M8.9 and ELDF10)

Size

£863.00

SKU:: R1475-10
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Goat
Immunogen:: Purified, E. coli-derived, recombinant human Renin R extracellular domain (rhRenin R; aa 17-302; Accession # O75787).
Format:: Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
Extra Details:: Renin R (Renin Receptor; also ATPase H + -transporting lysosomal accessory/interacting protein 2, M8.9 and ELDF10) is a 39-45kD protein that belongs to no known family. It is expressed on the surface of macrophages, vascular smooth muscle cells, renal mesangial cells and T cells. Renin R has at least two functions. First, it binds both renin and prorenin, promoting the conversion of angiotensinogen to angiotensin I. Second, its ligation induces PAI-1 synthesis. Mature human Renin R is a type I transmembrane protein 334 amino acids (aa) in length. It contains a 286 aa extracellular region (aa 17-302) and a 27 aa cytoplasmic tail. It is believed to form homodimers. Potential isoforms exist. One termed M8.9 shows a deletion of aa 1-252 and constitutes a component of the vacuolar proton ATPase. Two others show an alternate start site at Met77, and a deletion of aa 101-132. Over aa 17-302, the human Renin R shares 95% aa identity with the mouse Renin R. Applications: Suitable for use ELISA and Western Blot. Other applications not tested. Recommended Dilution: ELISA: 0.5-1.0ug/ml with the appropriate secondary reagents to detect human Renin R. The detection limit for rhRenin R is approximately 0.5ng/well Western Blot: 1ug/ml. Tissue lysates were resolved by SDS-PAGE, transferred to an Immobilon-P membrane and immunoblotted. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute by adding sterile 40-50% glycerol, aliquot and store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Shipping Conditions:: Blue Ice