R1424-95
Relaxin 1 (RLN1, Relaxin 1, H1, Prorelaxin H1, RLXH1)
Size
£1,004.00
- SKU:
- R1424-95
- Purity:
- Purified by Protein G affinity chromatography from cell culture supernatant.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rat
- Immunogen:
- E. coli-derived recombinant human Relaxin-1 (aa26-185)
- Clone:
- 8H157
- Format:
- Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 1ml sterile PBS.
- Extra Details:
- Human Relaxin-1/H1 is a 6kD member of the insulin/Relaxin gene superfamily. Relaxin-1 is synthesized as a 185 amino acid (aa) preprohormone that is proteolytically processed into a nonglycosylated, disulfide-linked, 53 aa mature heterodimer that contains a 29 aa B chain (aa 25-53) and a 24 aa A chain (aa 161-185). An alternate splice form of human Relaxin-2 is known that contains the B chain and an uncleaved 47 aa extension. Mature human Relaxin-1 heterodimer is 48%, 44% and 43% aa identical to rat, dog and pig Relaxin-2, respectively. Applications: Suitable for use in Direct ELISA, Western Blot and Neutralization of bioactivity. Other applications not tested. Recommended Dilutions: Western Blot: 1-2ug/ml. The detection limit is ~ 5ng/lane under non-reducing conditions. Neutralization: The ND50 is ~1-5ug/ml in presence of 100ng/ml of recombinant human Relaxin-1 using a c-AMP induction assay. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice