R1285-01

Recoverin, Recombinant, Human (RCVRN, Cancer Associated Retinopathy Protein, Cancer-associated Retinopathy Protein, CAR Protein, Protein CAR, RCV1)

Size

£448.00

SKU:: R1285-01
Application:: WB
Molecular Weight:: 23
Purity:: ≥95% by SDS-PAGE
Storage Conditions:: -20°C
Supplier:: United States Biological
Format:: Supplied as a liquid in 20mM Tris, pH 8.0, 1mM EDTA, 2mM MgCl2, 10% glycerol.
Extra Details:: Recoverin is a heterogeneously acylated calcium-binding and intracellular signal transduction protein in the photoreceptor cells of retina. Recoverin contains four EF-hands, of which two bind Ca. Ca-induced extrusion of the acyl group from a hydrophobic cleft in the protein drives the translocation of recoverin from solution to the disc membrane. Recently, recoverin is a detectable serologic protein that is expressed in patients with cancer-associated retinopathy, a paraneoplastic syndrome. Recombinant human recoverin was overexpressed in E. coli and purified by using conventional chromatography techniques. Amino Acid Sequence: MGNSKSGALS KEILEELQLN TKFSEEELCS WYQSFLKDCP TGRITQQQFQ SIYAKFFPDT DPKAYAQHVF RSFDSNLDGT LDFKEYVIAL HMTTAGKTNQ KLEWAFSLYD VDGNGTISKN EVLEIVMAIF KMITPEDVKL LPDDENTPEK RAEKIWKYFG KNDDDKLTEK EFIEGTLANK EILRLIQFEP QKVKEKMKNA Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.