Recombining Binding Protein Suppressor of Hairless (RBPSUH, CBF1, CBF-1, csl, IGKJRB, IGKJRB1, KBF2, J kappa-recombination Signal-binding Protein, RBP-J kappa, RBPJK, RBP-JK, RBPJ, RBP-J, Renal Carcinoma Antigen NY-REN-30, SUH)

Size

£581.00

SKU:: R1284-45B
Application:: WB
Purity:: Purified by Protein A and peptide affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Synthetic peptide corresponding to residues surrounding Glu111 of human RBPSUH.
Format:: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.
Extra Details:: RBPSUH (Recombining binding protein suppressor of hairless) is the DNA-binding component of the transcription complex regulated by Notch signaling. In the absence of Notch activation, RBPSUH suppresses target gene expression through interactions with a co-repressor complex containing histone deacetylase. Upon activation, the Notch intracellular domain (NICD) enters the nucleus and binds to RBPSUH to displace the co-repressor complex and replace with a transcription activation complex including Maml, histone acetylase p300, leading to the transcription of Notch target genes (1-3). In addition, RBPSUH is also the DNA-binding partner for Epstein-Barr virus (EBV) nuclear antigen 2 (EBNA2), a protein critical for latent viral transcription and immortalization of EBV-infected B cells (4,5). Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1:1000, Incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice