R1200-17A
Rab10 (RAB10, Member RAS Oncogene Family, Ras-related Protein Rab-10)
Size
£581.00
- SKU:
- R1200-17A
- Application:
- WB
- Purity:
- Purified by peptide affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Synthetic peptide corresponding to residues surrounding Asp114 of human Rab10 protein. Species Sequence Homology: rat (100%)
- Format:
- Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.
- Extra Details:
- Rab10 is a member of the Ras superfamily of small Rab GTPases (1) that interacts with Msst4, myosin V (Va, Vb and Vc), and GDI as it helps mediate sorting among cellular endosomes (2-4). Mutation analysis and GFP-fusion protein expression of Rab10 in MDCK cells determined that Rab10 plays a regulatory role in membrane protein transport between early endosome and basolateral compartments (5,6). Rab10 associates with the GLUT4 complex as a target for AS160 and is required for translocation of GLUT4 under insulin stimulation in adipocytes (7,8). Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1:1000, incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice