R1200-10E
Ras, R, Recombinant, Human (R-Ras, RRAS, Oncogene RRAS, p23, Ras-related Protein R-Ras, Related RAS Viral (r-Ras) Oncogene Homolog)
Size
£576.00
- SKU:
- R1200-10E
- Application:
- WB
- Molecular Weight:
- 25.3
- Purity:
- ~ 85% (SDS-PAGE). Purified by using conventional chromatography techniques.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Format:
- Supplied as a liquid in 20mM Tris HCl, pH 8.0, 0.1M NaCl, 10% glycerol.
- Extra Details:
- RRAS, also known as ras-related protein R-Ras, belongs to the Ras family which functions in signal transduction pathways. This protein has been shown to interact with RASSF5, NCK1, Bcl-2, ARAF and RALGDS. RRAS can bind GTP and GDP, and it has intrinsic GTPase activity. It also regulates the organization of the actin cytoskeleton. Recombinant RRAS protein, fused to His-tag at N-terminus, was expressed in E. coli. Source: Recombinant corresponding to aa1-215 of human RRAS 6x His tagged expressed in E. coli. Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MSSGAASGTG RGRPRGGGPG PGDPPPSETH KLVVVGGGGV GKSALTIQFI QSYFVSDYDP TIEDSYTKIC SVDGIPARLD ILDTAGQEEF GAMREQYMRA GHGFLLVFAI NDRQSFNEVG KLFTQILRVK DRDDFPVVLV GNKADLESQR QVPRSEASAF GASHHVAYFE ASAKLRLNVD EAFEQLVRAV RKYQEQELPP SPPSAPRKKG GGCPC Enzyme Activity: Not determined. This product is recommended for use in applications that do not require a catalytically active form of the protein. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice