R1198-35
Ras, H (Harvey Murine Sarcoma Virus Oncogene)
Size
£787.00
- SKU:
- R1198-35
- Purity:
- Purified
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rat
- Immunogen:
- Recombinant p21 ras protein. Epitope: aa62-76 of human ras.
- Clone:
- 3G260
- Format:
- Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.
- Extra Details:
- v-H-ras, K-ras and N-ras are 21kD phosphoproteins originally isolated based on their oncogenic properties and later found in all eukaryotic cells. Ras proteins belong to the Ras superfamily of monomeric GTPases which also includes the Rho, Rac, and Rab subfamilies. Ras proteins are anchored to the cytoplasmic face of plasma membranes where they function in switch-like fashion to relay signals from receptor tyrosine kinases to downstream effector molecules. Ras activation is regulated by the opposing effects of the activating guanine nucleotide exchange factors (GNEFs) that exchange ras-bound GDT with GTP and the inactivating GTPase accelerating proteins (GAPs). Ras activation of the MAP kinase signaling cascade is thought to occur via direct interaction of ras with the serine/thronine MAP-kinase-kinase-kinase raf. Applications: Suitable for use in Immunofluorescence, Western Blot, Immunoprecipitation, Immunohistochemistry and Neutralization. Other applications not tested. Recommended Dilutions: Optimal dilutions to be determined by the researcher. Positive Controls: SW480 or Y1 cells (IF and IP); normal skin (IHC, frozen and paraffin); A431, Hela or ML-1 cells (WB) Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice