R1171-01K-PE

Raptor (Regulatory Associated Protein of mTOR, p150 Target of Rapamycin (TOR) Scaffold Protein containing WD-repeats, KIAA1303) (PE)

Size

£1,010.00

SKU:: R1171-01K-PE
Purity:: Purified by Protein G affinity chromatography.
Storage Conditions:: 4°C Do Not Freeze
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Recombinant protein corresponding to human Raptor.
Clone:: 10F657 (1H6.2)
Format:: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).
Extra Details:: Raptor is a subunit of mTORC1 which regulates cell growth and survival in response to nutrient and hormonal signals. mTORC1 is activated in response to growth factors or amino-acids. Within mTORC1, Raptor positively regulates mTORC1 activity and functions as a scaffold for recruiting mTORC1 substrates. Applications: Suitable for use in Western Blot, Immunoprecipitation and Immunocytochemistry. Other applications not tested. Recommended Dilutions: Western Blot: 1ug/ml detected Raptor on 10ug of HEK293 cell lysate. HEK293 cell lysate was resolved by electrophoresis, transferred to PVDF membranes and probed with R1171-01K. Proteins were visualized using a goat anti-mouse IgG (HRP) and chemiluminescence detection system. Immunoprecipitation: 20ug Immunocytochemisty: 1: 500 detected Raptor in NIH/3T3 cells. Optimal dilutions to be determined by the researcher. Recommended Secondary Antibodies: I1904-06C: IgG, H&L (HRP) (X-Adsorbed) Pab Gt x Mo I1904-06H IgG, H&L (HRP) (X-Adsorbed) Pab Gt x Mo Positive Control: HEK 293 cell lysate. Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Note: Applications are based on unconjugated antibody.

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