R1171-01K-BIOTIN
Raptor (Regulatory Associated Protein of mTOR, p150 Target of Rapamycin (TOR) Scaffold Protein containing WD-repeats, KIAA1303) (Biotin)
Size
£1,010.00
- SKU:
- R1171-01K-BIOTIN
- Purity:
- Purified by Protein G affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Recombinant protein corresponding to human Raptor.
- Clone:
- 10F657 (1H6.2)
- Format:
- Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.
- Extra Details:
- Raptor is a subunit of mTORC1 which regulates cell growth and survival in response to nutrient and hormonal signals. mTORC1 is activated in response to growth factors or amino-acids. Within mTORC1, Raptor positively regulates mTORC1 activity and functions as a scaffold for recruiting mTORC1 substrates. Applications: Suitable for use in Western Blot, Immunoprecipitation and Immunocytochemistry. Other applications not tested. Recommended Dilutions: Western Blot: 1ug/ml detected Raptor on 10ug of HEK293 cell lysate. HEK293 cell lysate was resolved by electrophoresis, transferred to PVDF membranes and probed with R1171-01K. Proteins were visualized using a goat anti-mouse IgG (HRP) and chemiluminescence detection system. Immunoprecipitation: 20ug Immunocytochemisty: 1: 500 detected Raptor in NIH/3T3 cells. Optimal dilutions to be determined by the researcher. Recommended Secondary Antibodies: I1904-06C: IgG, H&L (HRP) (X-Adsorbed) Pab Gt x Mo I1904-06H IgG, H&L (HRP) (X-Adsorbed) Pab Gt x Mo Positive Control: HEK 293 cell lysate. Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.
- Shipping Conditions:
- Blue Ice