R1100-14A
RANTES (Regulated on Activation, Normal T-cell Expressed and Secreted) (Biotin)
Size
£479.00
- SKU:
- R1100-14A
- Purity:
- Purified by immunoaffinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Recombinant protein corresponding to mouse RANTES, expressed in E. coli.
- Format:
- Supplied as a lyophilized powder from sterile-filtered PBS, pH 7.2. Labeled with Biotin. Reconstitute with sterile PBS, 0.1% BSA to a concentration of 0.1-1.0mg/ml
- Extra Details:
- RANTES (regulated upon activation, normal T-cell expressed and secreted) is a 70-amino acid CC chemokine originally cloned from T cell-specific sequences-enriched cDNA libraries. Both human and murine RANTES are chemotactic for monocytes. Human RANTES is also a chemoattractant for T cells and eosinophils, as well as an activator of basophils for histamine release. In contrast to other CC chemokines, RANTES is only slowly expressed after activation of leukocytes. It is involved in a number of pathological conditions like chronic inflammation and autoimmune diseases. Application: Suitable for use in ELISA and Western Blot. Other applications have not been tested. Recommended Dilutions: ELISA: 0.25-1ug/ml (100ul/well). Detects 0.2ng/well of recombinant mRANTES. Western Blot (Reduced or non-reduced): 0.1-0.2ug/ml. Detects 1.5-3ng/lane of recombinant mRANTES. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS, 0.1% BSA. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice