R1100-02E

RANTES (Regulated on Activation Normal T-cell Expressed and Secreted, Regulated Upon Activation Normally T Expressed and Presumably Secreted, Beta Chemokine RANTES Precursor, CCL5, Chemokine (C-C Motif) Ligand 5, Chemokine CC Motif Ligand 5, D17S136E, MGC17164, SIS delta, SISd, Small Inducible Cytokine A5 (RANTES), Small Inducible Cytokine Subfamily A (Cys-Cys) Member 5, SCYA5, T cell Specific Protein p288, TCP228, T cell Specific RANTES Protein) (Biotin)

Size

£657.00

SKU:: R1100-02E
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Recombinant protein corresponding to human RANTES, expressed in E. coli.
Format:: Supplied as a lyophilized powder from PBS. No preservative added. Labeled with Biotin. Reconstitute with 500ul sterile PBS, 0.1% BSA.
Extra Details:: RANTES (regulated upon activation, normal T cell expressed and secreted), otherwise known as CCL5, a 68 amino acid CC- chemokine involved in T cell activation and cell recruitment, expressed by cytotoxic (CD8+) T cells (CTLs), macrophages, keratinocytes and endothelial cells. RANTES acts as a chemoattractant for monocytes, CD4+/CD45RO+ memory T cells, basophils and eosinophils, thorough binding to chemokine (C-C motif) receptor 1 (CCR1), CCR3 and CCR5. Both CCR3 and CCR5 are known co-receptors for the entry of HIV viruses into target cells and the binding of RANTES to CCR3 on CTLs has been shown to enhance HIV-1-specific cytotoxicity, whilst binding to CCR5 inhibits membrane fusion and hence HIV entry. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilutions: ELISA: 0.25-1ug/ml Western Blot: 0.1-0.2ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS, 0.1% BSA. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Shipping Conditions:: Blue Ice