R1100-01B
RANTES (Regulated on Activation, Normal T-cell Expressed and Secreted, CCL5)
Size
£629.00
- SKU:
- R1100-01B
- Purity:
- Purified by immunoaffinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Recombinant human RANTES, expressed in E. coli.
- Format:
- Supplied as a lyophilized powder from PBS, pH 7.2. Reconstitute in 100ul sterile ddH2O.
- Extra Details:
- RANTES (regulated upon activation, normal T-cell expressed and secreted) is a 70-amino acid CC chemokine originally cloned from T cell-specific sequences-enriched cDNA libraries. Both human and murine RANTES are chemotactic for monocytes. Human RANTES is also a chemoattractant for T cells and eosinophils, as well as an activator of basophils for histamine release. In contrast to other CC chemokines, RANTES is only slowly expressed after activation of leukocytes. It is involved in a number of pathological conditions like chronic inflammation and autoimmune diseases. Applications: Suitable for use in ELISA and Western Blot. Other applications have not been determined. Recommended Dilutions: ELISA (Sandwich): 0.5-2ug/ml. Using 100ul/well antibody solution. In conjunction with compatible secondary reagents, allows detection of 0.2-0.4ng/well of rhRANTES. Western Blot: 0.1-0.2ug/ml. Used in conjunction with compatible secondary reagents the detection limit for rhRANTES is 1.5-3.0ng/lane, under either reducing or non-reducing conditions. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice