R1075-07

RANK (Receptor Activator of NF-kB) (PE)

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£728.00

SKU:: R1075-07
Purity:: Purified by Protein G affinity chromatography
Storage Conditions:: 4°C Do Not Freeze
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Fusion protein containing amino acid residues 326-616 of human RANK. Epitope: aa330-727
Clone:: 4H238 (9A725)
Format:: Supplied as a liquid in PBS, 0.05% sodium azide. Labeled with R-Phycoerythrin (PE).
Extra Details:: Apoptosis, or programmed cell death, occurs during normal cellular differentiation and development of multicellular organisms. Apoptosis is induced by certain cytokines including TNF and Fas ligand of the TNF family through their death domain containing receptors, TNFR1 and Fas. Receptor activator of NF-kB (RANK) is a recently cloned member of the TNFR superfamily with no significant homology to other members of this family. RANK ligand (RANKL/TRANCE/ OPGL) binds to RANK on dendritic cells, upregulates the expression of anti-apoptotic protein BcL-XL suggesting a role in dendritic cell survival. The cytoplasmic domain of RANK interacts with TRAF2, TRAF5 and TRAF6. Overexpression of RANK activates NF-kB and c-Jun-terminal kinase (JNK) pathways. Recent studies have shown that RANK interaction with TRAF6 activates NF-kB, whereas JNK activation is mediated through binding of RANK to TRAF2. Applications: Suitable for use in Flow Cytometry, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Flow Cytometry (Cell surface and Intrcellular): 1ul to label 1x10e6 cells. Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1ug/ml for 30 minutes at RT. Western Blot: Band at ~97 using RAW cells Immunohistochemistry: 1:10-1:500 Immunohistochemistry: Paraffin sections Optimal dilutions to be determined by the researcher. Recommended Positive Control: RAW cells Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Shipping Conditions:: Blue Ice