R1073-BIOTIN
RanGAP 1 (Biotin)
Size
£971.00
- SKU:
- R1073-BIOTIN
- Purity:
- Purified by Protein A affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- A fusion protein consisting of the first 203 amino-terminal residues of the mouse RanGAP-1 protein.
- Clone:
- 3G259 (19C7)
- Format:
- Supplied as a liquid in PBS, pH 7.2. No preservatives added. Labeled with Biotin.
- Extra Details:
- Transport of macromolecules between the nucleus and cytoplasm occurs bi-directionally and is mediated by nuclear pore complexes (NPCs). NPCs are large supermolecular structures that span the nuclear envelope. Although ions, metabolites, and small macromolecules can passively diffuse through the NPC, most larger macromolecules such as proteins and ribonucleoproteins (RNPs) must be transported across the NPC by specific signal-and energy-dependent mechanisms. To date, the import of proteins into the nucleus has been studied in great detail and is considered to be the most well characterized aspect of nucleocytoplasmic transport. Nuclear import is specified by nuclear targeting or nuclear localization sequences (NLSs) in the imported protein. Applications: Suitable for use in Immunofluorescence, ELISA and Western Blot. Other applications not tested. Recommended Dilution: Immunofluorescence: 1ug/ml, see reference #8. ELISA: 0.1-1ug/ml Western Blot: 0.1-1ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.
- Shipping Conditions:
- Blue Ice