RAMP3 (Receptor Activity Modifying Protein3) (Not for Export EU)

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£704.00

SKU:: R1060-50A
Purity:: Serum
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: A 13 AA Peptide mapping at the middle and a 9 aa peptide mapping near the C-terminus of human RAMP3 (1) were selected for antibody production. The peptides were coupled to KLH
Format:: Supplied as a liquid, neat serum, 40% glycerol.
Extra Details:: The calcitonin family peptides probably act through G-protein coupled membrane receptors. The gene for calcitonin receptors has been cloned. It is homologous to GPCRs in family "B" which typically recognizes regulatory peptides (secretin, glucagons, VIP). Recently, a homolog of calcitonin receptor, CRLR (calcitonin-receptor-like receptor human 461 aa; rat/mouse 463 aa) was identified. CRLR has 55% homology with calcitonin receptor. It is now shown that CRLR can function as either a CGRP receptor or an ADM receptor, depending upon which members of a new family of proteins called receptor activity modifying proteins (RAMP1-3) are expressed. RAMPs1-3 contains an N-terminal signal peptide, an extracellular N-terminus, a single transmembrane domain near the C-terminus, and cytoplasmic C-terminus. RAMP1-3 displays 31% identity. RAMPS may be involved in the transport of CRLR to the plasma membrane. RAMP1 (human, mouse, rat 148 aa) presents the CRLR receptor as a glycoprotein that function as CGRP receptor. RAMP1 is expressed in many tissues, including the uterus, bladder, brain, pancreas, and GI tract. CRLR and RAMP1 are not co-expressed in all tissues suggesting that their co-expression may define which cells express functional CGRP receptors. RAMP2 (human 175 aa; rat 182 aa, and mouse 189 aa)-transported receptors are core-glycosylated and function as ADM receptor. It is expressed in the lung, breast, immune system and fetal tissues. RAMP3 is most abundant in the kidney and lung. Applications: Suitable for use in ELISA, Western Blotting. Other applications not tested. Recommended Dilution: Western Blotting 1:1000-1:5000 for neat serum and 1-10ug/ml for affinity pure antibody using ECL technique. ELISA: 1:10,000-50,000 for neat serum and 0.5-1ug/ml for affinity purified. Control peptide can be used to coat ELISA plates at 1ug/ml. Optimal dilutions to be determined by the researcher. Control Peptide: R10605-0C Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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