R1060-05C
RAMP2 (Receptor Activity Modifying Protein2)
Size
£825.00
- SKU:
- R1060-05C
- Purity:
- Purified by immunoaffinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Sheep
- Immunogen:
- Recombinant protein corresponding to Ser45-Val159 from mouse RAMP2 expressed in E. coli.
- Format:
- Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 500ul sterile PBS.
- Extra Details:
- RAMP2 is a 20kD member of the RAMP family of proteins. It is expressed on cardiomyocytes, vascular smooth muscle cells and endothelium, and interacts with CRLR to form a receptor complex for adrenomedullin (AM). AM induces vasodilation on AM1 receptor expressing cells. Mature mouse RAMP2 is a 145aa type I transmembrane glycoprotein that contains a 115aa extracellular domain (ECD) aa45-159 and a nine aa cytoplasmic region. Although the ECD contains no typical structural motifs, based on human, aa100-106 are critical for AM binding. Over aa45-159, mouse RAMP2 shares 57-83aa identity with human and rat RAMP2, respectively. Applications: Suitable for use in Flow Cytometry and Western Blot. Other applications not tested. Recommended Dilutions: Flow Cytometry: 2.5ug labels 10e6 cells Western Blot: 1ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice