R0495-09A
Raf, phosphorylated (Ser259) (Raf-1, c-Raf)
Size
£639.00
- SKU:
- R0495-09A
- Purity:
- Purified by Protein A affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Synthetic phosphopeptide corresponding to residues surrounding Ser259 of human c-Raf.
- Format:
- Supplied as liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. No preservative added.
- Extra Details:
- Raf-1, a mitogen-activated protein kinase, is the main effector recruited by GTP-bound Ras in order to activate the MAP kinase pathway (1). 14–3–3 binds to c-Raf at two phospho-serine residues: Ser259 and Ser621 (2). Mutation of Ser259 to alanine constitutively activates Raf kinase activity, but mutation of Ser621 to alanine results in an inactive kinase (2). Aktmediated phosphorylation of Ser259 has been shown in vivo to inhibit Raf kinase activity (4). Phosphorylation at Ser338 and Tyr341 of Raf-1 is a critical step for Raf-1 activation (5,6). p21-activated protein kinase (Pak) has been shown to phosphorylate Raf at Ser338 and to induce Raf-1 activity. Applications: Suitable for use in Western Blot and Immunoprecipitation. Other applications have not been tested. Recommended Dilution: Western Blot: 1:1000 Immunoprecipitation: 1:50 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice