R0495-01C

Raf1

Size

£245.00

SKU:: R0495-01C
Purity:: Purified by Protein G affinity chromatography.
Storage Conditions:: 4°C (-20°C Glycerol)
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Purified recombinant Raf-1 (75kD)
Clone:: 3H275
Format:: Supplied as a liquid in PBS, pH 7.2, 40% glycerol.
Extra Details:: Applications: Suitable for use in ELISA and Western Blot. Not suitable for the detection of Raf-1 expression and phosphorylation in HeLa cells. Other applications not tested. Recommended Dilution: Western Blot: 1ug/ml will allow visualization of 0.2ug/lane of human Raf-1. A main band (75kD) was visible. Optimal dilutions to be determined by the researcher. Myeloma: Sp2/0-Ag14 Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.