R0495-01A

Raf1

Size

£245.00

SKU:: R0495-01A
Purity:: Purified by Protein G affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Purified recombinant Raf-1 (75kD)
Clone:: 3H273 (540)
Format:: Supplied as a liquid in PBS, pH 7.2.
Extra Details:: Applications: Suitable for use in ELISA and Western Blot. Not suitable for detection of Raf-1 expression and phosphorylation in HeLa cells. Other applications not tested. Recommended Dilution: Western Blot: 1ug/ml will allow visualization of 0.2ug/lane of human Raf-1. A main band (75kD) was visible. Optimal dilutons to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.