R0451-01E-ML490
RAD51 (BRCC5, DNA Repair Protein RAD51 Homolog 1, E. coli RecA Homolog, HGNC:9817, HRAD51, HsRad51, HsT16930, RAD51, RAD51A, RecA Homolog (E. coli), RecA-like Protein) (MaxLight 490)
Size
£914.00
- SKU:
- R0451-01E-ML490
- Purity:
- Purified by Protein A chromatography
- Storage Conditions:
- 4°C Do Not Freeze
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Purified human RAD51 protein.
- Clone:
- 3C10
- Format:
- Supplied as a liquid in PBS, pH 7.2. No preservatives added. Labeled with MaxLight™490.
- Extra Details:
- MaxLight™ 490 is a new Blue-Green photostable dye conjugate comparable to DyLight™488, Alexa Fluor™488 and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (491nm); Emission (515nm); Extinction Coefficient 73,000. Rad51 is a structural and functional homolog of Escherichia coli RecA, which is required for genetic recombination, DNA repair, and cellular proliferation. This 43kD protein plays an important role in the repair of DNA double-strand breaks caused by ionizing radiation or from the breakdown of stalled replication forks. It forms helical nucleoprotein filaments in which the DNA lies extended and underwound. Filaments form preferentially on tailed duplex DNA substrates at the chromosomal break sites. Rad51 expression is increased in transformed cells and in cancer cell lines. Rad51 is overexpressed in breast carcinomas. Aberrant overexpression of Rad51 is directly correlated with radiation resistance and chemotherapeutic drug resistance. This aberrant expression of Rad51 is also related to enhanced recombination and genomic instabilities in breast carcinoma cells. Applications: Suitable for use in Western Blot and Immunoprecipitation. Other applications have not been tested. Recommended Dilution: Western Blot: 1-3ug/ml Immunoprecipitation: 10ug/IP Reaction Optimal dilutions to be determined by the researcher Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™490 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Note: Applications are based on unconjugated antibody.
- Shipping Conditions:
- Blue Ice