R0433-01

Rac2 (RAC2, ras-related C3 botulinum toxin substrate 2 (rho family, small GTP binding protein Rac2), EN-7, Ras-related C3 botulinum toxin substrate 2, rho family, small GTP binding protein Rac2, Ras-related C3 botulinum toxin substrate 3 (rho family, small GTP-binding protein Rac2))

Size

£642.00

SKU:: R0433-01
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Goat
Immunogen:: Synthetic peptide corresponding to amino acid sequence PQPTRQQKRACSLL on the C-terminus of human Rac2, NCBI accession #NP_002863.1.
Format:: Supplied as a liquid in Tris saline, pH 7.3, 0.5% BSA, 0.02% sodium azide.
Extra Details:: Applications: Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Peptide ELISA: 1:32,000 (detection limit) Western Blot: 1-3ug/ml. Approx 22kD band observed in lysates of Human T cell line MOLT4 (calculated MW of 21.4kD according to NP_002863.1). Staining (2ug/ml) of MOLT-4 lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by Western blot using chemiluminescence. Immunohistochemistry: 0.3ug/ml in paraffin embedded human tonsil; stains most cells in the mantle zone and interfollicular area. In the germinal centers the majority of cells are negative. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.