R0400

Rac1 (Ras-related C3 Botulinum Toxin Substrate 1 (Rho Family, Small GTP Binding Protein Rac1), Cell Migration-inducing Gene 5 Protein, MGC111543, MIG5, p21-Rac1, Ras-like Protein TC25, Ras-related C3 Botulinum Toxin Substrate 1, TC25, TC-25)

Size

£902.00

SKU:: R0400
Purity:: Purified by Protein G affinity chromatography from ascites.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Recombinant protein corresponding to the full-length human Rac1.
Clone:: 0.T.127
Format:: Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide, 30% glycerol.
Extra Details:: Ras related C3 botulinum toxin substrate 1 (RAC1) protein is a GTPase which belongs to the RAS superfamily of small GTP binding proteins. Members of this superfamily appear to regulate a diverse array of cellular events, including the control of cell growth, cytoskeletal reorganization, and the activation of protein kinases. Applications: Suitable for use in Western Blot, Immunohistochemistry, Affinity Precipitation and Immunoprecipitation. Other applications not tested. Recommended Dilutions: Western Blot: 0.5-2ug/ml. 20ug of rat brain microsomal prep was resolved by electrophoresis, transferred to PVDF or nitrocellulose and probed with R0400 (0.5ug/ml). Proteins were visualized using an IgG (HRP) goat anti-mouse and a chemiluminescence detection system. Immunohistochemistry (Frozen): 10ug/ml. Gave positive immunostaining of rat brain sections fixed first with 4% paraformaldehyde, 2% acetic acid in PBS for 30 minutes followed by ethanol:acetic acid [95:5] for 2 minutes. Affinity Precipitation Assay: 5-10ug of R0400-55A PAK-1 PBD agarose (as part of R0400-55 Rac1 Activation Assay Kit, BioAssay™) precipitated Rac1-GTP in lysates of 3T3 fibroblasts that were loaded in vitro with GTP-g-S. Proteins were detected by Western Blot anaylsis using 1ug/ml. Immunoprecipitation: 4ug immunoprecipitated Rac1 from a rat brain microsomal protein preparation. Optimal dilutions to be determined by the researcher. Positive Control: Western Blot/Immunoprecipitation: Rat brain microsomal protein preparation Immunohistochemistry: Rat brain sections Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice