R0400-05
Rac1/cdc42, phosphorylated (Ser71)
Size
£639.00
- SKU:
- R0400-05
- Purity:
- Purified by Protein A and peptide affinity chromatography
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Synthetic phosphopeptide corresponding to residues surrounding Ser71 of human Rac1/cdc42. Species Sequence Homology: mouse and rat, 100%
- Format:
- Supplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. No preservative added.
- Extra Details:
- Rac1 and cdc42 are members of the Rho-GTPase family. They play key roles in cytoskeletal reorganization, membrane trafficking, transcriptional regulation and cell growth and development. It has been established that GTP binding stimulates the activity of Rac1/cdc42. A putative Akt phosphorylation site at serine 71 of Rac1/cdc42 has been identified and confirmed by in vitro kinase assay (1). Phosphorylation at this site may inhibit GTP binding of Rac1, therefore attenuating the signal transduction pathway downstream of Rac1. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilutions: Western Blot: 1:1000. Incubate membrane with diluted antibody in 1X TBS, 5% BSA, 0.1% Tween-20 at 4°C with gentle shaking, overnight. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice