R0400-02B
Rac1 (Ras-related C3 Botulinum Toxin Substrate 1 (Rho Family, Small GTP binding protein Rac1), Cell migration-inducing gene 5 protein, MGC111543, MIG5, p21-Rac1, Ras-like Protein TC25, Ras-related C3 Botulinum Toxin Substrate 1, TC-25)
Size
£825.00
- SKU:
- R0400-02B
- Application:
- WB
- Purity:
- Purified by Protein A affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Synthetic peptide corresponding to aa130-141 of human Rac1 (KEKKLTPITYPQ-C). Cellular Localization: Cell membrane; Lipid-anchor; Cytoplasmic side.
- Format:
- Supplied as a liquid in phosphate, 0.15M sodium chloride, pH 7.6, 0.09% sodium azide.
- Extra Details:
- Rac is a member of the Rho family of small GTP-binding proteins, and plays roles in cytoskeletal actin organization as well as transformation. Rac is activated downstream of cdc42, or independently by Ras signaling, and in turn Rac stimulates Rho. Activation of Rac results in the formation of lamellipodia, whereas cdc42 stimulates filopodia formation, and Rho stimulates formation of actin stress fibers. Effectors of the Rac pathway include NADPH Oxidase and the SAPK1/JNK family of kinases. The latter pathway is activated by the PAK kinases, which are direct targets of Rac. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1:500-1:1000 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice