R0009-85

RAB27A, aa1- 221, Recombinant, Human (GTP-binding Protein Ram, MGC117246, RAB27, Rab-27, RAM, Ras-related Protein Rab-27A)

Size

£453.00

SKU:: R0009-85
Molecular Weight:: 27
Purity:: ~90% (SDS-PAGE).
Storage Conditions:: -20°C
Supplier:: United States Biological
Format:: Supplied as a liquid in 20mM Tris-HCl, pH 8.0, 1mM DTT, 20% glycerol.
Extra Details:: Ras-related protein Rab-27A, also known as RAB27A, belongs to the small GTPase superfamily, Rab family. The protein is membrane-bound and may be involved in protein transport and small GTPase mediated signal transduction. Also, RAB27A regulates the cytotoxic granule exocytosis and affect T-lymphocyte and macrophage-activation. It is widely expressed with significant expression in pancreatic islets and pituitary tissue, and low expression in brain. Defects in RAB27A cause Griscelli syndrome type 2 (GS2). Source: Recombinant corresponding to aa1-221 of human RAB27A 6x his tagged, expressed in E.coli. AA Sequences: MGSSHHHHHH SSGLVPRGSH MSDGDYDYLI KFLALGDSGV GKTSVLYQYT DGKFNSKFIT TVGIDFREKR VVYRASGPDG ATGRGQRIHL QLWDTAGQER FRSLTTAFFR DAMGFLLLFD LTNEQSFLNV RNWISQLQMH AYCENPDIVL CGNKSDLEDQ RVVKEEEAIA LAEKYGIPYF ETSAANGTNI SQAIEMLLDL IMKRMERCVD KSWIPEGVVR SNGHASTDQL SEEKEKGACG Molecular Weight: 27kD (241aa), confirmed by MALDI-TOF Enzyme Activity: Not determined. This product is recommended for use in applications that do not require a catalytically active form of the protein. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing.. Store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Shipping Conditions:: Blue Ice