P9544-15

Pyruvate Dehydrogenase E1-alpha Subunit, phosphorylated (Ser293) (Pyruvate Dehydrogenase E1 alpha, Pyruvate Dehydrogenase E1 Component Subunit alpha Somatic Form Mitochondrial, PDH, PDHCE1A, PDHE1-A Type I, PHE1A, Pyruvate Dehydrogenase (Lipoamide) alpha 1, PDHA1)

Size

£626.00

SKU:: P9544-15
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Synthetic peptide surrounding the phosphorylated serine 293 of the human PDHE1 alpha protein.
Format:: Supplied as a liquid in Tris-citrate/phosphate, pH 7.5, 0.09% sodium azide.
Extra Details:: The pyruvate dehydrogenase complex (PDC) is a nuclear-encoded mitochondrial matrix multienzyme complex that provides the primary link between glycolysis and the tricarboxylic acid (TCA) cycle by catalyzing the irreversible conversion of pyruvate into acetyl-CoA. The PDH complex is composed of multiple copies of 3 enzymes: E1 (PDHA1 or PDHE1), dihydrolipoyl transacetylase (DLAT), and dihydrolipoyl dehydrogenase (DLD). The E1 enzyme is a heterotetramer of 2 alpha and 2 beta subunits. The E1-alpha subunit contains the E1 active site and plays a key role in the function of the PDH complex. Enhanced expression of pyruvate dehydrogenase kinase-1 (PDK-1) results in phosphorylation of PDHE1 alpha at Serine 293 which in turn produces nearly complete inhibition of the pyruvate dehydrogenase complex (PDC). A recent study shows that inhibition of PDC activity in cancer cells restores normoxic stablization of HIF-1 alpha by glycolytic metabolites. Applications: Suitable for use in Immunofluorescence/Immunocytochemistry and Western Blot. Other applications not tested. Recommended Dilutions: Western Blot: 1:1000-1:5000 detects a band at ~43kD. Immunofluorescence (IC): 1:50-1:250 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice