P9543-95A
Pyruvate Carboxylase (PC, PCB, Pyruvate carboxylase, mitochondrial, Pyruvic carboxylase)
Size
£642.00
- SKU:
- P9543-95A
- Purity:
- Purified by immunoaffinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Goat
- Immunogen:
- Synthetic peptide corresponding to C-KFKEVKKAYVEANQ from an internal region of human PC.. Species Sequence Homology: rat, canine, mouse, bovine and porcine
- Format:
- Supplied as a liquid in Tris saline, pH 7.3, 0.5% BSA, 0.02% sodium azide.
- Extra Details:
- This gene encodes pyruvate carboxylase, which requires biotin and ATP to catalyse the carboxylation of pyruvate to oxaloacetate. The active enzyme is a homotetramer arranged in a tetrahedron which is located exclusively in the mitochondrial matrix. Pyruvate carboxylase is involved in gluconeogenesis, lipogenesis, insulin secretion and synthesis of the neurotransmitter glutamate. Mutations in this gene have been associated with pyruvate carboxylase deficiency. Alternatively spliced transcript variants with different 5' UTRs, but encoding the same protein, have been found for this gene. Applications: Suitable for use in Peptide ELISA, Flow Cytometry and Immunohistochemistry Other applications not tested. Recommended Dilutions: Peptide ELISA: 1:16000. Immunohistochemistry (FFPE): 5ug/ml in human liver Flow Cytometry: 10ug/ml in HeLa cells Optimal dilutions to be determined by the researcher. Positive Conrol: Mouse Liver lysates Control Peptide: P9543-95A-P: Pyruvate Carboxylase (PC, PCB, Pyruvate carboxylase, mitochondrial, Pyruvic carboxylase) (Control Peptide) Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice