P9452-45
Pygopus Homolog 1 (Pygopus-1, PYGO1)
Size
£761.00
- SKU:
- P9452-45
- Application:
- WB
- Purity:
- Purified by Protein G affinity chromatography from hybridoma culture supernatant.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- E.coli-derived recombinant protein corresponding to Ala2-Ala419 from human Pygopus-1.
- Clone:
- 11C46
- Format:
- Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 200u.l sterile PBS.
- Extra Details:
- Human Pygopus-1 (PYGO1, the name of a legless Australian lizard) is a 45kD, 419aa transcriptional coactivator. It contains four domains: an N-terminal NLS (aa35-41), followed by a Pro-rich, Asn-rich, and zinc-finger PHD-type domain (aa340-398). It is a nuclear protein that acts in concert with BCL-9 to retain b-catenin in the nucleus during Wnt-signaling. In particular, following b-catenin activation and translocation to the nucleus, b-catenin first binds to BCL-9 in a phosphorylation-independent manner. Its continued presence in the nucleus depends upon BCL-9 binding to Pygopus. Once anchored, b-catenin interacts with TCF to activate Wnt-responsive genes. Human Pygopus-1 is 87% aa identical to mouse Pygopus-1. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilutions: Western Blot: 1ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice