PUMA (p53 Upregulated Modulator of Apoptosis, BCL2 Binding Component 3, BCL-2 Binding Component 3, BBC3, JFY1, JFY-1, p53 Up-regulated Modulator of Apoptosis, PUMA alpha, PUMA/JFY1)

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£594.00

SKU:: P9198-02
Application:: WB
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: A synthetic peptide made to a region within the C-terminus of the human PUMA protein sequence (between residues 150-193).
Format:: As reported
Extra Details:: A novel gene PUMA (p53 upregulated modulator of apoptosis) is a target for activation by the p53 tumor-suppressor gene. p53 functions as a transcriptional activator, and influences p53-inducible genes that play a role in the induction of apoptosis in response to p53. Antisense inhibition of PUMA expression reduced the apoptotic response to p53, and PUMA is likely to play a role in mediating p53-induced cell death through the cytochrome c/Apaf-1-dependent pathway. Positive Control: HL-60 whole cell lysate Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.