PTF1A (Pancreas Transcription Factor 1 Subunit alpha, Class A Basic Helix-loop-helix Protein 29, bHLHa29, Pancreas-specific Transcription Factor 1a, bHLH Transcription Factor p48, p48 DNA-binding Subunit of Transcription Factor PTF1, PTF1-p48, BHLHA29, PTF1P48)

Size

£642.00

SKU:: P9182-42G
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Goat
Immunogen:: Synthetic peptide corresponding to C-WTDEKQLKEQN, from PTF1A, at the internal region of the protein (NP_835455.1).
Format:: Supplied as a liquid in Tris saline, pH 7.3, 0.5% BSA, 0.02% sodium azide.
Extra Details:: Transcriptional activator. Binds to the E-box consensus sequence 5'-CANNTG-3'. Plays an important role in determining whether cells allocated to the pancreatic buds continue towards pancreatic organogenesis or revert back to duodenal fates. May be involved in the maintenance of exocrine pancreas-specific gene expression including ELA1 and amylase. Required for the formation of pancreatic acinar and ductal cells. Plays an important role in cerebellar development. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: ELISA: 1:32,000 Western Blot: 0.5-1.5ug/ml, observed in human brain (cerebellum) lysates on ~48kD bands Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.