P9182-05H
PTEN Tumor Suppressor Protein (MMAC1, Phosphatase and Tensin, TEP1)
Size
£672.00
- SKU:
- P9182-05H
- Purity:
- Purified by immunoaffinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Synthetic peptide corresponding to aa254-270 (KVEFFHKQNKMLKKDKM) of human PTEN. Species sequence homology: human, chimpanzee, mouse, rat and canine.
- Format:
- Supplied as a liquid in TBS, pH 7.4, 0.1% BSA, 0.02% sodium azide, 50% glycerol.
- Extra Details:
- PTEN (phosphatase and tensin homology on chromosome 10) dephosphorylates proteins and lipids such as AKT and phosphatidylinositol 3,4,5-triphosphate (PIP3) and therefore functions as a key regulatory enzyme in a central signal transduction pathway. It also interacts with proteins Shc and FAK to promote apoptosis when necessary and to regulate cell division and migration.1 PTEN is considered a tumor suppressor as loss-of-function mutations in PTEN often result in human cancers including melanoma and prostate carcinoma.2,3 PTEN is expressed in almost all tissues of the body as a 403 amino acid protein with an estimated molecular weight of 47kD. Applications: Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: 2ug/ml Immunohistochemistry (Formalin-fixed paraffin-embedded sections): 2ug/ml Optimal dilutions to be determined by the researcher. Positive Controls: A431 and HEK293 Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice