P9182-03
PTEN Tumor Suppressor Protein (MMAC1, Phosphatase and Tensin, TEP1)
Size
£1,151.00
- SKU:
- P9182-03
- Application:
- IHC-Fr
- Purity:
- Tissue culture supernatant
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Recombinant protein corresponding to human PTEN.
- Clone:
- 0.N.540B (17.A)
- Format:
- Supplied as a liquid in 0.09% sodium azide.
- Extra Details:
- PTEN is a candidate tumor suppressor gene mapping to the homozygous deletion on human chromosome 10q23. It appears to be mutated at considerable frequency in human cancers. The predicted PTEN protein has a protein tyrosine phosphatase domain and extensive homology to tensin. Studies have shown that purified PTEN catalyzes dephosphorylation of PtdIns(3,4,5)P3, specifically at position 3 on the inositol ring, and raise the possibility that PTEN acts in vivo as a phosphoinositide 3-phosphatase by regulating PtdIns(3,4,5)P3 levels. PTEN has been implicated in a large number of human tumors and is conserved from humans to worms. Characterization of PTEN protein showed that it is a phosphatase that acts on proteins and on 3-phosphorylated phosphoinositides, and can therefore modulate signal-transduction pathways that involve lipid second messengers. Recent results indicate that at least part of its role is to regulate the activity of the serine/threonine kinase AKT/PKB, and thus influence cell survival signaling. Although PTEN has the consensus sequence of a protein tyrosine phosphatase, it dephosphorylates p-nitrophenylphosphate and other synthetic and protein substrates poorly. The greatest catalytic activity has been observed with the highly negatively charged, multiply phosphorylated polymer of (Glu-Tyr)n. Applications: Suitable for use in Immunohistochemistry. Other applications not tested. Recommended Dilution: Immunohistochemistry (FFPE): 1:25–1:50 for 30 min at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min. Optimal dilutions to be determined by the researcher. Cellular Localization: Cytoplasmic Positive Control: Prostate carcinoma. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice