P9182-02A-ML550
PTEN Tumor Suppressor Protein Tumor Suppressor Protein (MMAC1, Phosphatase and Tensin, TEP1) (MaxLight 550)
Size
£914.00
- SKU:
- P9182-02A-ML550
- Purity:
- Purified by Protein A chromatography
- Storage Conditions:
- 4°C Do Not Freeze
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Recombinant protein derived from the PTEN protein.
- Clone:
- 2F4C9
- Format:
- Supplied as a liquid in PBS, pH 7.2. No preservatives added. Labeled with MaxLight™550.
- Extra Details:
- PTEN (also know as MMAC1) was recently identified as a candidate tumor suppressor gene. Deletions or mutations within the PTEN gene have been observed in a variety of tumor cell lines and cancer cell types. Based on PTEN’s structural features, the protein was originally thought to be a protein tyrosine phosphatase. However, recent evidence indicates that PTEN is actually a lipid phosphatase rather that a protein tyrosine phosphatase. In fact, the target substrate for PTEN is phosphatidylinositol-3,4,5-triphosphate (Ptd-Ins(3,4,5)P3). Ptd-Ins(3,4,5)P3 is a critical second messenger involved in cell signaling and growth control. Ptd-Ins(3,4,5)P3 is produced by phosphorylation of Ptd-Ins(4,5)P2 by PI 3-kinase in response to stimulation by various ligands. By dephosphorylating Ptd-Ins(3,4,5)P3, PTEN functions to “turn off” the Ptd-Ins(3,4,5)P3 pathway and there by suppress cellular proliferation. Applications: Suitable for use in Western Blot and FLISA. Other applications have not been tested. Recommended Dilution: Western Blot: 1-3ug/ml FLISA: 0.1-1.0ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™550 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Note: Applications are based on unconjugated antibody.
- Shipping Conditions:
- Blue Ice