P9182-02A-FITC

PTEN Tumor Suppressor Protein Tumor Suppressor Protein (MMAC1, Phosphatase and Tensin, TEP1) (FITC)

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£914.00

SKU:: P9182-02A-FITC
Purity:: Purified by Protein A chromatography
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Recombinant protein derived from the PTEN protein.
Clone:: 2F4C9
Format:: Supplied as a liquid in PBS, pH 7.2. No preservatives added. Labeled with Fluorescein isothiocyanate (FITC).
Extra Details:: PTEN (also know as MMAC1) was recently identified as a candidate tumor suppressor gene. Deletions or mutations within the PTEN gene have been observed in a variety of tumor cell lines and cancer cell types. Based on PTEN’s structural features, the protein was originally thought to be a protein tyrosine phosphatase. However, recent evidence indicates that PTEN is actually a lipid phosphatase rather that a protein tyrosine phosphatase. In fact, the target substrate for PTEN is phosphatidylinositol-3,4,5-triphosphate (Ptd-Ins(3,4,5)P3). Ptd-Ins(3,4,5)P3 is a critical second messenger involved in cell signaling and growth control. Ptd-Ins(3,4,5)P3 is produced by phosphorylation of Ptd-Ins(4,5)P2 by PI 3-kinase in response to stimulation by various ligands. By dephosphorylating Ptd-Ins(3,4,5)P3, PTEN functions to “turn off” the Ptd-Ins(3,4,5)P3 pathway and there by suppress cellular proliferation. Applications: Suitable for use in Western Blot and FLISA. Other applications have not been tested. Recommended Dilution: Western Blot: 1-3ug/ml FLISA: 0.1-1.0ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.

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