P9162-BIOTIN
P-Selectin, Glycoprotein Ligand-1 (PSGL 1) (Biotin)
Size
£984.00
- SKU:
- P9162-BIOTIN
- Purity:
- Purified
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- The antibody epitope was mapped to a site within a consensus tyrosine sulfation motif of PSGL-1, previously shown to be essential for interaction with P-selectin (and now shown to be essential for recognition of PSGL-1 by L-selectin).
- Clone:
- 2Q745 (KPL-1)
- Format:
- Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.
- Extra Details:
- Interactions between P-selectin and P-selectin glycoprotein ligand-1 (PSGL-1) mediate the earliest “rolling” of leukocytes on the lumenal surface of endothelial cells at sites of inflammation. To date, the primary role of PSGL-1 in mediating the interaction between neutrophils and P-selectin has been well documented. Two color Flow Cytometry of normal leukocytes has demonstrated that PSGL-1 is expressed on essentially all blood neutrophils, NK cells, blood cells, T cells, and monocytes, but PSGL-1 stains B cells at significantly lower levels than other cell types. Variation in tyrosine sulfation during B cell differentiation may affect the stability of B cells to interact with P- and L-selectin. Applications: Suitable for use in FACS, Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.
- Shipping Conditions:
- Blue Ice