P9150-15

Postsynaptic Density Protein 95 CT (PSD-95, PSD95, Discs Large Homolog 4, DLG4, Human Post-synaptic Density Protein 95, Synapse-associated Protein 90, SAP90, SAP-90)

Size

£728.00

SKU:: P9150-15
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Synthetic peptide corresponding to the C-terminal region of human PSD-95, conjugated to KLH.
Format:: Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.
Extra Details:: PSD-95 belongs to a class of proteins referred to as MAGUKs (membrane associated guanylate kinases). Cannonical MAGUK proteins contain one or three PDZ (PSD-95/discs large/zona occludens-1) domains, an SH3 domain, and a guanylate kinase homology domain. PSD-95 and several other MAGUK and PDZ containing proteins (eg. PSD-93, SAP-97, SAP-102, MALS/Veli proteins(2)) are enriched in the post synaptic density.(3) These proteins appear to regulate synaptic function by acting as molecular scaffolds to organize and cluster signaling machinery at synapses, in part by interaction of their PDZ domains with carboxy-terminal T/SXV motifs present on synaptic proteins including NMDA receptors and Shaker type K+ channels. PSD-95 has also been shown to link NMDA receptors to the downstream signal transduction protein neuronal nitric oxide synthetase (nNOS) via a novel PDZ:PDZ interaction with nNOS. Applications: Suitable for use in Immunofluorescence, ELISA, Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilutions: Immunofluorescence: 0.5-1.0ug/ml ELISA: 0.1-1ug/ml Western Blot: 1ug/ml Immunoprecipitation: 5ug/IP reaction Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice