P9150-15-ML490
Postsynaptic Density Protein 95 CT (PSD-95, PSD95, Discs Large Homolog 4, DLG4, Human Post-synaptic Density Protein 95, Synapse-associated Protein 90, SAP90, SAP-90) (MaxLight 490)
Size
£984.00
- SKU:
- P9150-15-ML490
- Purity:
- Purified by immunoaffinity chromatography.
- Storage Conditions:
- 4°C Do Not Freeze
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Synthetic peptide corresponding to the C-terminal region of human PSD-95, conjugated to KLH.
- Format:
- Supplied as a liquid in PBS, pH 7.2. No preservatives added. Labeled with MaxLight™490.
- Extra Details:
- MaxLight™ 490 is a new Blue-Green photostable dye conjugate comparable to DyLight™488, Alexa Fluor™488 and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (491nm); Emission (515nm); Extinction Coefficient 73,000. PSD-95 belongs to a class of proteins referred to as MAGUKs (membrane associated guanylate kinases). Cannonical MAGUK proteins contain one or three PDZ (PSD-95/discs large/zona occludens-1) domains, an SH3 domain, and a guanylate kinase homology domain. PSD-95 and several other MAGUK and PDZ containing proteins (eg. PSD-93, SAP-97, SAP-102, MALS/Veli proteins(2)) are enriched in the post synaptic density.(3) These proteins appear to regulate synaptic function by acting as molecular scaffolds to organize and cluster signaling machinery at synapses, in part by interaction of their PDZ domains with carboxy-terminal T/SXV motifs present on synaptic proteins including NMDA receptors and Shaker type K+ channels. PSD-95 has also been shown to link NMDA receptors to the downstream signal transduction protein neuronal nitric oxide synthetase (nNOS) via a novel PDZ:PDZ interaction with nNOS. Applications: Suitable for use in Immunofluorescence, FLISA, Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilutions: Immunofluorescence: 0.5-1.0ug/ml FLISA: 0.1-1.0ug/ml Western Blot: 1ug/ml Immunoprecipitation (Rat): 5ug/IP reaction Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™490 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Note: Applications are based on unconjugated antibody.
- Shipping Conditions:
- Blue Ice