P9109-40L
Protein Tyrosine Phosphatase Receptor Type M (PTPRM, PTPu, PTPRu, and RPTPu)
Size
£825.00
- SKU:
- P9109-40L
- Purity:
- Purified by Protein A affinity chromatography from hybridoma culture supernatant.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Recombinant protein corresponding to Glu21-Lys742 from human PTPRM, expressed in NS0 cells.
- Clone:
- 8B41
- Format:
- Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 200ul sterile PBS.
- Extra Details:
- Protein Tyrosine Phosphatase, receptor type M (PTPRM), also called PTPμ, PTPRμ, and RPTPμ, is expressed at highest levels in pulmonary vascular epithelia, where interactions with cadhedrins are believed to be important in regulating barrier permeability. The MAM and Fibronectin III (FNIII) domains on the extracellular side of PTPRM bind between cells, affecting adhesion and contact inhibition. Culturing cells to high density concentrates PTPRM at sites of tight contact and induces proteolytic cleavage of the 100kD extracellular domain. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilutions: Western Blot: 1ug/ml under reducing conditions with HepG2 human hepatocellular carcinoma cell line, and CHP-100 human neuroblastoma cell line. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice