P9109-27Y
Protein Tyrosine Phosphatase, Non-receptor Type 5 (PTPN5, Striatum-Enriched Protein-Tyrosine Phosphatase, STEP, Striatal enriched phosphatase, FLJ14427)
Size
£960.00
- SKU:
- P9109-27Y
- Purity:
- Purified by Protein G affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Synthetic peptide corresponding to aa57-74, MCTPGCNEEGFGYLVSPR, of rat STEP46 conjugated to KLH. Species Sequence Homology: Mouse (100%), human (94%)
- Clone:
- 5H41 (23E5)
- Format:
- Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide, 30% glycerol.
- Extra Details:
- STEP (Protein-tyrosine phosphatase striatum-enriched) is a neural-specific protein-tyrosine phosphatase. It is also known as PTPN5 (tyrosine-protein phosphatase, non-receptor type 5). STEP immunoreactive areas are most intense in the regions of the CNS receiving a dopaminergic input (e.g. striatum, basal ganglia). STEP is part of the NMDA receptor (R) complex and inhibits NMDAR single-channel activity and NMDAR-mediated synaptic current. Phosphorylation of STEP, via D1 and PKA activation by dopamine, decreases the activity of STEP by reducing its affinity for substrate. Applications: Suitable for use in Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Western Blot: 1:1000-1:10,000 detected STEP in RIPA lysates from whole rat brain preparations. Whole rat brain preparations were resolved by electrophoresis, transferred to nitrocellulose and probed with diluted P9109-27Y. Proteins were visualized using an IgG (HRP) Pab Gt xMo* and a chemiluminescence detection system. Immunoprecipitation: 4ug immunoprecipitated STEP from 500ug of whole rat brain preparations. Immunohistochemistry: Reported by an independent laboratory to detect STEP in rat brain sections. Optimal dilutions to be determined by the researcher. *Recommended Secondary Antibodies: I1904-06C: IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo I1904-06H IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice