P9108-16

Protein serine/threonine phosphatase 2C alpha/beta

Size

£736.00

SKU:: P9108-16
Purity:: Purified by ammonium sulfate precipitation
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Synthetic peptide corresponding to NH2-Arg-Tyr-Gly-Leu-Ser-Ser-Met-Gly-Trp-Arg-Val-Glu-COOH, conjugated to KLH
Format:: Supplied as a liquid in PBS, 0.08% sodium azide.
Extra Details:: Type 2 protein phosphatases are serine/threonine phosphatases which can preferentially dephosphorylate the alpha subunit of phosphorylase kinase and are insensitive to inhibitor 2 treatment. Protein phosphatase 2A (PP2A) can be distinguished from PP2B and PP2C which require Ca2+ and Mg2+, respectively, by its partial activity in the absence of divalent cations. PP2A activity is potently inhibited by treatment with okadaic acid and shows much stronger activity toward histone phosphorylated by Protein Kinase C and casein phosphorylated by Protein Kinase A than other protein phosphatases. Applications: Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilutions: Western Blot: 5-10ug/ml Immunoprecipitation: 5-10ug/ml Optimal dilutions to be determined by researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.