P9107-34A
Protein Phosphatase 2AB (PP2AB)
Size
£812.00
- SKU:
- P9107-34A
- Purity:
- Ascites
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Synthetic peptide corresponding to aa398-411, CASGKRKKDEISVD of human PP2A, B subunit (KLH). Preferential recognition for b isoforms as follows: a, d > b > g. Species sequence homology: rabbit, rat, bovine, porcine and Xenopus.
- Clone:
- 1.T.17 (2G9)
- Format:
- Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide, 30% glycerol.
- Extra Details:
- Applications: Suitable for use in Western Blot and Immunoprecipitation. Other applications have not been tested. Recommended Dilutions: Western Blot: 1:1000 dilution detects PP2A, B subunit in RIPA lysates from non-stimulated A431 cells and mouse 3T3/A31 cells. Non-stimulated A431 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with P9107-34A, PP2A, B subunit (1:1000 dilution) Proteins were visualized using an IgG (HRP) goat anti-mouse and a chemiluminescence detection system. Immunoprecipitation: Immunoprecipitates the B subunit of PP2A. Optimal dilutions to be determined by the researcher. Recommended Secondary Antibodies: I1904-06C: IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo I1904-06H IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo I1904-14C: IgG, Fc (HRP) Pab Gt xMo I1904-19C: IgG, F(ab’)2 (HRP) Pab Gt xMo Included Positive Antigen Control: A0001-50 Non stimulated A431 lysate. Add 2.5ul of 2-mercaptoethanol/100ul of lysate and boil for 5 minutes to reduce the preparation. Load 20ug of reduced lysate per lane for minigels. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice