P9107-33A-ML650
Protein Phosphatase 2A, Serine, Threonine (PP2A Ser/Thr) (MaxLight 650)
Size
£1,070.00
- SKU:
- P9107-33A-ML650
- Purity:
- Purified by Protein A affinity chromatography.
- Storage Conditions:
- 4°C Do Not Freeze
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Synthetic peptide corresponding to aa296-309 within the C-terminus of human protein phosphatase 2A, conjugated to KLH.
- Format:
- Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™650.
- Extra Details:
- MaxLight™650 is a new Far-IR stable dye conjugate comparable to Alexa Fluor™647, DyLight™649, Cy5™ and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (655nm); Emission (676nm); Extinction Coefficient 250,000. Type 2 protein phosphatases are serine/threonine phosphatases which can preferentially dephosphorylate the alpha subunit of phosphorylase kinase and are insensitive to inhibitor 2 treatment. Protein phosphatase 2A (PP2A) can be distinguished from PP2B and PP2C which require Ca2+ and Mg2+, respectively, by its partial activity in the absence of divalent cations. PP2A activity is potently inhibited by treatment with okadaic acid and shows much stronger activity toward histone phosphorylated by Protein Kinase C and casein phosphorylated by Protein Kinase A than other protein phosphatases. Applications: Suitable for use in Western Blot, Immunoprecipitation and Phosphatase assays. Other applications have not been tested. Recommended Dilutions: Western Blot: Detects PP2A catalytic subunit (~34kD) in rabbit brain membrane cytosol and in human A431 RIPA cell lysate. Immunoprecipitation: immunoprecipitates PP2A catalytic subunit from 500ug rabbit brain extract and 1mg human A431 RIPA cell lysate. Immunoprecipitation/Phosphatase Assay: 5ug immunoprecipitates PP2A from 1U of purified Protein Phosphatase 2A, Serine, Threonine (PP2A Ser/Thr). Phospatase activity was demonstrated using p-Nitrophenyl Phosphate (pNPP, KR-pT-IRR), Ser/Thr Phosphatase Assay Kit, BioAssay™ (Catalog No: N2600-35). Optimal dilutions to be detemined by the researcher. Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Note: Applications are based on unconjugated antibody.
- Shipping Conditions:
- Blue Ice