P9107-32

Protein Phosphatase 2A, demethylated (PP2A)

Size

£767.00

SKU:: P9107-32
Purity:: Ascites
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Synthetic peptide corresponding to aa295-309, CRGEPHVTRRTPDYFL of human PP2A, C subunit, with cysteine added to facilitate conjugation (KLH). Species Sequence Homology: mouse, rat, bovine, porcine, rabbit and chicken.
Clone:: 4i57 (4b7)
Format:: Supplied as a liquid 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide, 30% glycerol.
Extra Details:: Type 2 protein phosphatases are serine/threonine phosphatases which can preferentially dephosphorylate the a subunit of phosphorylase kinase and are insensitive to inhibitor 2 treatment. Protein phosphatase 2A (PP2A) can be distinguished from PP2B and PP2C which require Ca 2+ and Mg 2+ , respectively, by its partial activity in the absence of divalent cations. PP2A activity is potently inhibited by treatment with okadaic acid and shows much stronger activity toward histone phosphorylated by Protein Kinase C and casein phosphorylated by Protein Kinase A than other protein phosphatases. Applications: Suitable for use in ELISA, Immunoprecipitation and Western Blot. Other applications not tested. Recommended Dilutions: Immunoprecipitation: Reported to immunoprecipitate unmethylated PP2A, C subunit. Western Blot: 1000-10,000 detects PP2A, demethylated in RIPA lysates from A431 cells. A431 cell lysate was resolved by electrophoresis, transferred to PVDF and probed with P9107-32 (1:5000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. Optimal dilutions to be determined by the researcher. Included Positive Control: A0001-50: Non-stimulated A431 cell lysate. Add 2.5ul of 2-mercaptoethanol/100ul of lysate and boil for 5 minutes to reduce the preparation. Load 20ug of reduced lysate per lane for immunoblot analysis. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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