P9107-13

Protein Phosphatase 2A, B56 gamma (PP2g)

Size

£709.00

SKU:: P9107-13
Application:: WB
Purity:: Purified by Protein A affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Full-length human PP2A B56 gamma fusion protein, which is 90% homologous with mouse and rat.
Clone:: 7C5
Format:: Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.
Extra Details:: Protein phosphatase type 2A (PP2A) is a major eukaryotic serine/threonine protein phosphatase (PPase). PP2A is highly conserved and often exist as a heterotrimeric enzyme composed of a catalytic (C) subunit, a structural (A) subunit, and a variable regulatory/targeting (B-type) subunit1. Recent studies show that PP2A plays important roles in cell cycle regulation,signal transduction, cell differentiation, and transformation1-3. Its activity is also related to several diseases, including neurodegenerative diseases and cancer. The enzymatic activity and intracellular localization of PP2A are determined by three distinct families of cellular regulatory subunits, B, B’, and B’’. The B’ subunit, also known as B56, is most diverse, consisting of five isoforms (alpha, beta, gamma, delta, and epsilon).4,5 Based on a conserved epitope, shared with the B’ subunits, striatin (PR110) and S/G2 nuclear autoantigen (SG2NA; PR93) were identified as new members of a potential B’’’ subunit family. A recent study demonstrated that PP2A B56 gamma subunits binds both APC and cyclin G, and that suppression of this PP2A B subunit may directly perturb specific signaling pathways. Alterations in PP2A B56 gamma subunit function may contribute to cancer development in spontaneously arising human tumors.6 Applications: Suitable for use in Western Blot. Other applications have not been tested. Recommended Dilution: Western Blot: 1-3ug/ml. Detects a band of ~60kD in human PP2A B56γ-transfected 293T cell lysates. Optimal working dilutions to be determined by researcher. Positive Control: PP2A B56gamma-transfected 293T cell lysates. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice