P9107-10A

Protein Phosphatase 1, alpha (PP1a)

Size

£581.00

SKU:: P9107-10A
Purity:: Purified by Protein A affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Synthetic peptide (KLH coupled) corresponding to the N-terminal residues of human PP1 alpha.
Format:: Supplied as liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. No preservative added.
Extra Details:: Type 1 protein phosphatase (PP1), a serine/threonine phosphatase, is highly conserved in eukaryotic cells. Four isoforms of PP1 have been characterized: PP1 alpha, PP1 delta, PP1 gamma1 and PP1 gamma2 (1). One of the biological functions of PP1 is involved in the cell cycle regulation (1). It has been illustrated that PP1 dephosphorylates pRB and cdc25 during mitosis (2,3). The activity and specificity of PP1 is highly regulated by regulatory subunits and targeting proteins (4). A cellcycle dependent phosphorylation at threonine 320 of PP1 alpha by cdc2 kinase has been reported recently. This phosphorylation inhibits PP1 alpha activity (5). Recommended Dilution: Western Blotting: 1:1000 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.