P9104-91K
Podoplanin (PDPN, Aggrus, Glycoprotein 36kD, Glycoprotein 36, gp36, PA2.26 Antigen, T1-alpha, T1A, PSEC0003, PSEC0025) (Biotin)
Size
£876.00
- SKU:
- P9104-91K
- Application:
- WB
- Purity:
- Purified by immunoaffinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Sheep
- Immunogen:
- Recombinant protein corresponding to Glu21-Lys123 of human Podoplanin expressed in NSO cells.
- Format:
- Supplied as a lyophilized powder from PBS with 50ug/1ug BSA. Labeled with Biotin. Reconstitute with 250ul sterile PBS.
- Extra Details:
- Podoplanin, also called PA2.26 antigen, gp38, aggrus or T1a, is a type1 transmembrane sialomucin-type glycoprotein. It is specifically expressed by lymphatic endothelial cells but not blood vascular endothelial cells. Various carcinoma cells, as well as epithelial cells, keratinocytes and neuronal cells can also express the protein. Podoplanin is involved in cell adhesion and motility. The sialylated o-glycans on Podoplanin have been found to be required for Podoplanin-induced platelet aggregation. As a consequence of alternative splicing, an alternative isoform for human Podoplanin has been reported. Within the sequence included in this immunogen, human podoplanin shares 32% aa sequence identity with mouse podoplanin. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilutions: Western Blot: 0.1ug/ml using recombinant human Podoplanin Fc Chimera Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice