P9104-91-BIOTIN

Podoplanin (PDPN, Aggrus, Glycoprotein 36kD, Glycoprotein 36, gp36, GP38, HT1A-1, hT1alpha1, hT1alpha2, Lung Type-I Cell Membrane-associated Glycoprotein Isoform a, OTS8, OTS-8, PA2.26 Antigen, T1 alpha, T1A, TIA2) (Biotin)

Size

£927.00

SKU:: P9104-91-BIOTIN
Application:: IHC-Fr
Purity:: Purified by Protein G affinity chromatography from tissue culture supernatant.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: ORF Human podoplanin ectodomaine expressed in E. coli.
Clone:: 6A457 (4D5aE5E6)
Format:: Supplied as a liquid in PBS, pH 7.2. Labeled with Biotin.
Extra Details:: Podoplanin is absent on blood endothelium and therefore this product is readily able to distinguish between lymphatic vessels and blood vasculature. Although the specific function of this protein is undetermined, Podoplanin is reported to be a reliable indicator of tumor lymphangiogenesis. Applications: Suitable for use in Immunohistochemistry. Other applications not tested. Recommended Dilution: Immunohistochemistry (Frozen and paraffin): 0.75-2ug/ml Note: Requires antigen retrieval using heat treatment prior to staining of paraffin sections. Use sodium citrate buffer, pH 6.0. Optimal dilution determined by the researcher. Recommended Secondary Reagents: I1904-65W: IgG, F(ab’)2 (HRP) Pab RbxMo I1904-66C: IgG, F(ab’)2, X-Adsorbed (HRP) Pab RbxMo Recommended Negative Control: I1904-78R: IgG1 Murine Negative Control Recommended Positive Control Tissue: Normal lung Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.

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