P9104-87B
Podocalyxin
Size
£889.00
- SKU:
- P9104-87B
- Purity:
- Purified by Protein A affinity chromatography from hybridoma culture supernatant
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Recombinant protein corresponding to Ser23-Arg427 from human Podocalyxin, expressed in NS0 cells.
- Clone:
- 8H121
- Format:
- Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 200ul sterile PBS.
- Extra Details:
- Podocalyxin, also known as Podocalyxin-like protein-1 (PCLP1 or PODXL), is a type I transmembrane glycoprotein. It belongs to the CD34/Podocalyxin family of sialomucins that share structural similarity and sequence homology (1). Podocalyxin is a major sialoprotein in the podocytes of the kidney glomerulus and is also expressed by both endothelium and multipotent hematopoietic progenitors (2). It has been identified as a novel cell surface marker for hemangioblasts, the common precursors of hematopoietic and endothelial cells. Applications: Suitable for use in Flow Cytometry, Immunocytochemistry and Western Blot. Other applications have not been tested. Recommended Dilutions: Flow Cytometry: 0.25ug labels 10e6 BG01V human embryonic stem cells Immunocytochemistry: 8-25ug/ml using fixed BG01V human embryonic stem cells Western Blot: 1ug/ml using human recombinant Podocalyxin Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice