P9103-72Q3
PKN1 (Serine/Threonine-protein Kinase N1, Protease-activated Kinase 1, AK-1, Protein Kinase C-like 1, Protein Kinase C-like PKN, Protein Kinase PKN-alpha, Protein-kinase C-related Kinase 1, PAK1, PKN, PRK1, PRKCL1, Serine-threonine Protein Kinase N, ) (Not for Export EU)
Size
£460.00
- SKU:
- P9103-72Q3
- Purity:
- Serum
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Synthetic peptide corresponding to aa911-929 of human PKN (EAPTLSPPRDARPLTAAEQ).
- Format:
- Supplied as a liquid, 0.05% sodium azide.
- Extra Details:
- Protein Kinase 1 (PKN) belongs to the protein kinase C superfamily. This kinase is activated by Rho family of small G proteins and may mediate the Rho-dependent signaling pathway. This kinase can be activated by phospholipids and by limited proteolysis. The 3-phosphoinositide dependent protein kinase-1 (PDPK1/PDK1) is reported to phosphorylate this kinase, which may mediate insulin signals to the actin cytoskeleton. The proteolytic activation of this kinase by caspase-3 or related proteases during apoptosis suggests its role in signal transduction related to apoptosis. Alternatively spliced transcript variants encoding distinct isoforms have been observed. Applications: Suitable for use in Western Blot, Immunohistochemistry and Immunoprecipitation. Other applications not tested. Recommended Dilution: Western Blot: 1:1000-1:2000 Immunohistochemistry (formalin fixed paraffin embedded): 1:1000-1:5000 Immunoprecipitation: 1:50-1:200 Immunohistochemistry: Frozen Optimal dilutions to be determined by the researcher. Positive Control: Spleen, lymphoid, CA cell lines Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice