P9103-40E

Protein Kinase C, zeta, Mid (PKCz)

Size

£704.00

SKU:: P9103-40E
Purity:: Purified by immunoaffinity chromatography
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Synthetic peptide derived from the middle region of the catalytic domain of the mouse protein kinase C, zeta isoform (PKCz) protein.
Format:: Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.
Extra Details:: PKC isoforms are divided into three classes: conventional (cPKC), novel and atypical (aPKC).1 Vertebrates express two atypical PKCs, z and i/l. PKCi2 and -l3 are human and mouse orthologues, respectively. PKCz ia a key regulator of signaling pathways, and is involved in the MAP kinase cascade, NFkB activation, ribosomal S6-protein kinase signaling, and cell polarity.Brain-specific PKCz, or PKMz, is a new PKC isoform, synthesized from PKMz mRNA encoding a PKCz catalytic domain without a regulatory domain, which is not a proteolytic fragment of PKCz, as previously thought. PKMz is a specific, autonomously active form of aPKCz4 that is both necessary and sufficient for maintaining hippocampal long-term potentiation (LTP). PKCz is the only PKC isozyme with a stable PKM form in hippocampus.6,7 Metabolic labeling of hippocampal slices shows increased de novo synthesis of PKMz in LTP. Because PKMz is a kinase synthesized in an autonomously active form and is necessary and sufficient for maintaining LTP, it serves as an example of a link coupling gene expression directly to synaptic plasticity. Applications: Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilutions: Western Blot: 1-3ug/ml Immunoprecipitation: 7ug/IP reaction Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice